Dehydrogenation of Sterols by the Protozoan Tetrahymena pyriformis
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چکیده
منابع مشابه
Two-dimensional gravitactic bioconvection in a protozoan (Tetrahymena pyriformis) culture.
Gravitactic bioconvective patterns created by Tetrahymena pyriformis in a Hele-Shaw apparatus were realized and compared with theoretical results. There were found to be two thresholds for bio-convection development: the first indicates the transition from the diffusion to the steady convection state; the second corresponds to the transition from the steady to the unsteady convection state. The...
متن کاملThe steric requirements for the metabolism of sterols by Tetrahymena pyriformis.
Cultures of Tetrahymena pyriformis were incubated with a series of sterols which differed from one another in the steric character of their side chains. (E)-1X20)-Dehydrocholesterol in which C-22 is fixed to the right (bans with respect to C-13, “right-handed”) in the usual view of the molecule was converted to its A5,7,17(2”’ and A5v7,17(20)22 derivatives; however, the organism did not signifi...
متن کاملSteric effects at C-20 and C-24 on the metabolism of sterols by Tetrahymena pyriformis.
Cultures of Tetrahymena pyriformis were incubated with various sterols and the extent of dehydrogenation at C-7 and C-22 was determined. The sterols incubated were desmosterol, 22-dehydrodesmosterol, 24-methyldesmosterol, 24 alpha-methylcholesterol (campesterol), 24-methylene-cholesterol, isohalosterol (26,27-bisnorcampesterol, also known as 24,24-dimethylchol-5-en-e beta-ol, a naturally occurr...
متن کاملBiomethylation and Volatilization of Arsenic by Model Protozoan Tetrahymena pyriformis under Different Phosphate Regimes
Tetrahymena pyriformis, a freshwater protozoan, is common in aquatic systems. Arsenic detoxification through biotransformation by T. pyriformis is important but poorly understood. Arsenic metabolic pathways (including cellular accumulation, effluxion, biomethylation, and volatilization) of T. pyriformis were investigated at various phosphate concentrations. The total intracellular As concentrat...
متن کاملAutomated image analysis to improve bead ingestion toxicity test counts in the protozoan Tetrahymena pyriformis.
AIMS To improve bead ingestion counts in Tetrahymena pyriformis by automated image analysis as an alternative to direct-counts. METHODS AND RESULTS Fluorescent latex beads were added to T. pyriformis cultures for ingestion tests. The number of beads ingested by 25 cells was counted directly by epifluorescence microscopy and compared with similar data from image analysis. anova indicated that ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1971
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)34148-1